Danny Cipriani, Chris Ashton and Luther Burrell have all been omitted from England's provisional 45-man elite player squad for a pre-season summit.
Fly-half Cipriani and winger Ashton were not involved as England whitewashed Australia 3-0 in June's Test series, but centre Luther Burrell featured in the first match in Brisbane and is perhaps the most surprising omission.
Ashton was initially included by head coach Eddie Jones for this year's Six Nations but was ruled out of the tournament through suspension and later rejected a call from the England Saxons side to tour South Africa.
Cipriani, who will re-join Wasps for next season, has not played for England since a World Cup warm-up against France last August and was found guilty of drink-driving in June.
However, Wasps' Fiji-born forward Nathan Hughes is included, while Leicester Tigers hooker Tom Youngs - surprisingly overlooked for the Six Nations - is called up for the first time by Jones.
Jonny May is included after the Gloucester winger recovered from a serious knee injury, while Dan Robson, Mike Haley and Charlie Ewels – part of the Saxons' tour to South Africa – are named, as are Joe Marchant and Will Evans, who were part of the squad that won the World U-20 Championship this year.
According to Jones, the camp is crucial for England's preparation ahead of the November and December internationals, which start against South Africa on November 12.
"This camp sets the scene for November," Jones told the RFU's official website. "It's important to catch up with the players prior to the start of the season and continue to put our plans in place.
"We'll use this time to reset and review each player's goals so they're clear on what is expected of them between now and when we meet up again in October.
"It also gives us an opportunity to meet and assess some new players and ensure they are familiar with the England setup.
"The long-term strategy for England is to develop a side who can be the most dominant team in world rugby, so we've chosen a number of new faces to attend this camp on the form they have shown recently or the potential we see in them."